Human Gene WIPF2 (uc002hug.1) (2024)

Human Gene WIPF2 (uc002hug.1)

Description: hom*o sapiens WAS/WASL interacting protein family, member 2 (WIPF2), mRNA.
RefSeq Summary (NM_133264): This gene encodes a WASP interacting protein (WIP)-related protein. It has been shown that this protein has a role in the WASP-mediated organization of the actin cytoskeleton and that this protein is a potential link between the activated platelet-derived growth factor receptor and the actin polymerization machinery. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
Position:hg19 chr17:38,375,574-38,438,439Size:62,866Total Exon Count:8Strand:+
Coding Region
Position:hg19 chr17:38,412,712-38,434,477Size:21,766Coding Exon Count:7

Page Index	Sequence and Links	UniProtKB Comments	Primers	CTD	Gene Alleles
RNA-Seq Expression	Microarray Expression	RNA Structure	Protein Structure	Other Species	GO Annotations
mRNA Descriptions	Pathways	Other Names	Model Information	Methods

Data last updated at UCSC:2013-06-14

Sequence and Links to Tools and Databases

Genomic Sequence (chr17:38,375,574-38,438,439)			mRNA (may differ from genome)		Protein (440 aa)
Gene Sorter	Genome Browser	Other Species FASTA	VisiGene	Gene interactions	Table Schema
AlphaFold	BioGPS	Ensembl	Entrez Gene	ExonPrimer	GeneCards
GeneNetwork	H-INV	HGNC	Lynx	MGI	neXtProt
OMIM	PubMed	Reactome	Treefam	UniProtKB	Wikipedia
BioGrid CRISPR DB

Comments and Description Text from UniProtKB


	ID: WIPF2_HUMAN DESCRIPTION: RecName: Full=WAS/WASL-interacting protein family member 2; AltName: Full=WASP-interacting protein-related protein; AltName: Full=WIP- and CR16-homologous protein; AltName: Full=WIP-related protein; FUNCTION:* Plays an active role in the formation of cell surface protrusions downstream of activated PDGFB receptors. Plays an important role in actin-microspike formation through cooperation with WASL. May cooperate with WASP and WASL to induce mobilization and reorganization of the actin filament system. SUBUNIT: Interacts with WASL and WASP, and this interaction results in cytoplasmic relocation of these two proteins along actin filaments. Interacts with NCK2 resulting in the localization to sites of focal adhesions. No interaction was seen with WASF2 and WASF3. INTERACTION: P62993:GRB2; NbExp=3; IntAct=EBI-2850112, EBI-401755; SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Note=Localized to stress fibers and bundles of actin filaments. TISSUE SPECIFICITY: Expressed mainly in brain, colon, lung and stomach (at protein level). Ubiquitously expressed, with high expression in brain, kidney, lung, and placenta. MISCELLANEOUS: Access to the profilin-binding site is masked in the full-length protein. SIMILARITY: Belongs to the verprolin family. SIMILARITY: Contains 1 WH2 domain. SEQUENCE CAUTION: Sequence=AAQ15232.1; Type=Frameshift; Positions=373;

Primer design for this transcript


	Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA. Click here to load the transcript sequence and exon structure into Primer3Plus Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence. Click here to open Exonprimer with this transcript To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3

Comparative Toxicogenomics Database (CTD)


	The following chemicals interact with this gene D001564Benzo(a)pyrene C044387N,N,N',N'-tetrakis(2-pyridylmethyl)ethylenediamine D013749Tetrachlorodibenzodioxin D019287Zinc Sulfate

Common Gene Haplotype Alleles


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RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)


	Highest median expression: 13.25 RPKM in Brain - Frontal Cortex (BA9) Total median expression: 368.34 RPKM View in GTEx track of Genome BrowserView at GTEx portalView GTEx Body Map

Highest median expression: 13.25 RPKM in Brain - Frontal Cortex (BA9)
Total median expression: 368.34 RPKM

View in GTEx track of Genome BrowserView at GTEx portalView GTEx Body Map

Microarray Expression Data


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mRNA Secondary Structure of 3' and 5' UTRs

Region	Fold Energy	Bases	Energy/Base	Display As
5' UTR	-90.02	240	-0.375	Picture	PostScript	Text
3' UTR	-1364.35	3962	-0.344	Picture	PostScript	Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

Protein Domain and Structure Information

InterPro Domains: Graphical view of domain structure
IPR003124 - WH2_dom

Pfam Domains:
PF02205 - WH2 motif

ModBase Predicted Comparative 3D Structure on Q8TF74


Front	Top	Side

The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

Orthologous Genes in Other Species

Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.

Mouse	Rat	Zebrafish	D. melanogaster	C. elegans	S. cerevisiae
No ortholog	No ortholog	No ortholog	No ortholog	No ortholog	No ortholog
Gene Details
Gene Sorter

Gene Ontology (GO) Annotations with Structured Vocabulary


	Molecular Function: GO:0003779 actin binding GO:0005515 protein binding Biological Process: GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis Cellular Component: GO:0005654 nucleoplasm GO:0005737 cytoplasm GO:0005829 cytosol GO:0005856 cytoskeleton GO:0005886 plasma membrane

Molecular Function:
GO:0003779 actin binding
GO:0005515 protein binding

Biological Process:
GO:0038096 Fc-gamma receptor signaling pathway involved in phagocytosis

Cellular Component:
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005886 plasma membrane

Descriptions from all associated GenBank mRNAs


	AK289779 - homo sapiens cDNA FLJ78696 complete cds, highly similar to homo sapiens WIRE protein (WIRE), mRNA. AK311544 - homo sapiens cDNA, FLJ18586. AL834239 - homo sapiens mRNA; cDNA DKFZp547N082 (from clone DKFZp547N082). BC025965 - homo sapiens cDNA clone IMAGE:5458710. AK315836 - homo sapiens cDNA, FLJ79485 complete cds, highly similar to WIP-related protein. AK290301 - homo sapiens cDNA FLJ77749 complete cds, highly similar to homo sapiens WIRE protein (WIRE), mRNA. AK055057 - homo sapiens cDNA FLJ30495 fis, clone BRAWH2000323, moderately similar to WIP-related protein. BC065551 - homo sapiens WAS/WASL interacting protein family, member 2, mRNA (cDNA clone MGC:70467 IMAGE:6044222), complete cds. AF370396 - homo sapiens PP10631 mRNA, complete cds. AK289578 - homo sapiens cDNA FLJ75102 complete cds, highly similar to homo sapiens WIRE protein (WIRE), mRNA. LF326319 - JP 2014500723-A/133822: Polycomb-Associated Non-Coding RNAs. JC506674 - Sequence 42 from Patent EP2733220. JC737786 - Sequence 42 from Patent WO2014075939. JC506661 - Sequence 29 from Patent EP2733220. JC737773 - Sequence 29 from Patent WO2014075939. JC506688 - Sequence 56 from Patent EP2733220. JC737800 - Sequence 56 from Patent WO2014075939. JC506666 - Sequence 34 from Patent EP2733220. JC737778 - Sequence 34 from Patent WO2014075939. MA561896 - JP 2018138019-A/133822: Polycomb-Associated Non-Coding RNAs. AB043786 - homo sapiens mRNA for WICH, complete cds. AJ431177 - homo sapiens mRNA for WIRE protein. AK026913 - homo sapiens cDNA: FLJ23260 fis, clone COL05804, highly similar to HSU90911 Human clone 23652 mRNA sequence. BC043408 - homo sapiens WIRE protein, mRNA (cDNA clone IMAGE:6064801), partial cds. U90911 - Human clone 23652 mRNA sequence. JD164812 - Sequence 145836 from Patent EP1572962. JD450065 - Sequence 431089 from Patent EP1572962. JD040589 - Sequence 21613 from Patent EP1572962. JD091033 - Sequence 72057 from Patent EP1572962. JD126466 - Sequence 107490 from Patent EP1572962. JD352339 - Sequence 333363 from Patent EP1572962. JD036939 - Sequence 17963 from Patent EP1572962. JD538693 - Sequence 519717 from Patent EP1572962. JD039427 - Sequence 20451 from Patent EP1572962. JD133503 - Sequence 114527 from Patent EP1572962. JD204224 - Sequence 185248 from Patent EP1572962. JD306507 - Sequence 287531 from Patent EP1572962. JD390372 - Sequence 371396 from Patent EP1572962. JD207510 - Sequence 188534 from Patent EP1572962. JD036865 - Sequence 17889 from Patent EP1572962. JD412562 - Sequence 393586 from Patent EP1572962. JD422579 - Sequence 403603 from Patent EP1572962. JD263105 - Sequence 244129 from Patent EP1572962. JD300322 - Sequence 281346 from Patent EP1572962. JD530678 - Sequence 511702 from Patent EP1572962. JD547513 - Sequence 528537 from Patent EP1572962. DQ586635 - homo sapiens piRNA piR-53747, complete sequence. JD071848 - Sequence 52872 from Patent EP1572962. JD137061 - Sequence 118085 from Patent EP1572962. JD372813 - Sequence 353837 from Patent EP1572962. JD186473 - Sequence 167497 from Patent EP1572962. JD019112 - Sequence 136 from Patent EP1572962. JD034563 - Sequence 15587 from Patent EP1572962. BC019706 - homo sapiens cDNA clone IMAGE:4513687, partial cds. AK055025 - homo sapiens cDNA FLJ30463 fis, clone BRACE2009517. BC047435 - homo sapiens cDNA clone IMAGE:5313516. BC064504 - homo sapiens cDNA clone IMAGE:6537819, partial cds. JD148351 - Sequence 129375 from Patent EP1572962. JD249926 - Sequence 230950 from Patent EP1572962. JD511594 - Sequence 492618 from Patent EP1572962. JD319667 - Sequence 300691 from Patent EP1572962. JD447282 - Sequence 428306 from Patent EP1572962. JD149213 - Sequence 130237 from Patent EP1572962. JD149215 - Sequence 130239 from Patent EP1572962. JD054482 - Sequence 35506 from Patent EP1572962. JD106509 - Sequence 87533 from Patent EP1572962. JD044561 - Sequence 25585 from Patent EP1572962. DQ598333 - hom*o sapiens piRNA piR-36399, complete sequence.

Biochemical and Signaling Pathways


	Reactome (by CSHL, EBI, and GO) Protein Q8TF74 (Reactome details) participates in the following event(s): R-HSA-2197691 Activation of WASP/N-WASP by WIP family and SH3 domain proteins R-HSA-2197698 Phosphorylation of WASP/N-WASP R-HSA-442592 WASPs or WAVEs activate the ARP2/3 complex R-HSA-2197690 Detachment of WASP/WAVE R-HSA-2029466 Attachment of preexisting mother filament and initiation of branching R-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs R-HSA-2029480 Fcgamma receptor (FCGR) dependent phagocytosis R-HSA-195258 RHO GTPase Effectors R-HSA-168249 Innate Immune System R-HSA-194315 Signaling by Rho GTPases R-HSA-168256 Immune System R-HSA-162582 Signal Transduction

Reactome (by CSHL, EBI, and GO)

Protein Q8TF74 (Reactome details) participates in the following event(s):

R-HSA-2197691 Activation of WASP/N-WASP by WIP family and SH3 domain proteins
R-HSA-2197698 Phosphorylation of WASP/N-WASP
R-HSA-442592 WASPs or WAVEs activate the ARP2/3 complex
R-HSA-2197690 Detachment of WASP/WAVE
R-HSA-2029466 Attachment of preexisting mother filament and initiation of branching
R-HSA-2029482 Regulation of actin dynamics for phagocytic cup formation
R-HSA-5663213 RHO GTPases Activate WASPs and WAVEs
R-HSA-2029480 Fcgamma receptor (FCGR) dependent phagocytosis
R-HSA-195258 RHO GTPase Effectors
R-HSA-168249 Innate Immune System
R-HSA-194315 Signaling by Rho GTPases
R-HSA-168256 Immune System
R-HSA-162582 Signal Transduction

Other Names for This Gene


	Alternate Gene Symbols: A8K0L3, NM_133264, NP_573571, PP10631, Q658J8, Q71RE1, Q8TE44, Q8TF74, WICH, WIPF2_HUMAN, WIRE UCSC ID: uc002hug.1 RefSeq Accession: NM_133264 Protein: Q8TF74 (aka WIPF2_HUMAN) CCDS: CCDS11364.1

Gene Model Information

category:	coding	nonsense-mediated-decay:	no	RNA accession:	NM_133264.4
exon count:	8	CDS single in 3' UTR:	no	RNA size:	5525
ORF size:	1323	CDS single in intron:	no	Alignment % ID:	100.00
txCdsPredict score:	2156.50	frame shift in genome:	no	% Coverage:	100.00
has start codon:	yes	stop codon in genome:	no	# of Alignments:	1
has end codon:	yes	retained intron:	no	# AT/AC introns	0
selenocysteine:	no	end bleed into intron:	0	# strange splices:	0

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Methods, Credits, and Use Restrictions


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